I currently study genetic interations among Colorado pinyon pine (Pinus edulis) populations and their associated dependent communities at Sunset Crater National Monument near Flagstaff, AZ. Various pinyon pines and juniper (Juniperus spp.) species comprise the pinyon-juniper woodland that is widespread across the Southwest United States. Colorado pinyon pines are the most abundant of the pinyons in this system and are presently under considerable drought stress (see figure below).
Over the past 30 years, NAU researchers have identified the population of P. edulis at Sunset Crater NM to exhibit differential morphologies relating to their efficiency of water use, tolerance to drought, and resistance to herbivory. As of July 2010 I have developed several simple sequence repeat (SSR) genetic markers for use in this species (publication pending) and will use them to study the dynamics of P. edulis on the Colorado Plateau and in regions where P. edulis may be hybridizing with closely related pine species. Additionally, I will address the soil microbial communities (bacteria, fungi, mycorrhizal fungi) through quantitative terminal restriction fragment length polymorphism to assess the importance of various microorganisms in the success or failure of individual P. edulis genotypes. These studies will help identify elements of the P. edulis genome under selection and microbial community members important to the health of pinyon-juniper woodland facing an uncertain future given the changing climate.
Protocols I regularly use:
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Publications and symposia:
K Kesler, A Krohn*, P Polsgrove*, C Roe*, C Ueckert (2010). Bringing Biotechnology into the Classroom: 3 Biotechnology Lessons You Can Use Next Week. NSF Graduate STEM Fellows in K-12 Education Annual Projects Meeting, 26-28 March, 2010, Washington D.C. *presenting authors.
H Riday & AL Krohn (2010). Genetic Map-Based Location of the Red Clover (Trifolium pratense L.) Gametophytic Self-incompatibility Locus. Theoretical and Applied Genetics. Published online 12 May, 2010, DOI: 10.1007/s00122-010-1347-0.
H Riday & AL Krohn (2010). Increasing population hybridity by restricting self-incompatibility alleles in red clover. Crop Science 50:853-860.
MD Casler, MM Phillips & AL Krohn (2009). DNA polymorphisms reveal geographic races of reed canarygrass. Crop Science 49:2139-2148.
J Knack*, A Krohn & H Riday (2008). Increasing hybridity via control of the gametophytic self-incompatibility system in red clover (Trifolium pretense). UW Undergraduate Research Symopsium, Madison, WI. *presenting author
LM Winton, AL Krohn & RH Leiner (2007). Microsatellite markers for Sclerotinia subarctica nom. Prov., a new vegetable pathogen of the High North. Molecular Ecology Notes 7:1077-1079.
LM Winton, AL Krohn & JS Conn (2007). Microsatellite markers for the invasive plant species white sweetclover (Melilotus alba) and yellow sweetclover (Melilotus officinalis). Molecular Ecology Notes 7:1296-1298.
LM Winton, RH Leiner, AL Krohn & KL Deahl, (2007). Occurrence of late blight caused by Phytophthora infestans on potato and tomato in Alaska. Plant Disease 91:634.
LM Winton, RH Leiner & AL Krohn ( 2006). Genetic diversity of Sclerotinia species from Alaskan vegetable crops. Canadian Journal of Plant Pathology 28:426-434.
LM Winton, RH Leiner & AL Krohn (2006). Hybridization and recombination in exotic and endemic Sclerotinia species in Alaska. Phytopathology 96:S124.
AL Krohn*, JA Bickmeier & FG Plumley (2004). Genome analysis of a saxitoxin-producing cyanobacterium. Presented at: XI International Conference on Harmful Algal Blooms, 14-19 Nov, 2004, Cape Town, S.A. *presenting author
Resources I regularly use:
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